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. 2013 Jul;29(6):539–549. doi: 10.1089/jop.2012.0179

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 4. — Terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL). No TUNEL-positive cell was observed at 1 and 7 days after the sham operation (sham 1d, a; Sham 7d, d). After ischemia and pretreatment with vehicle (I/R 1 d+Veh, b; I/R 7 d+Veh, e) or 0.5 nmol baicalein (I/R 1 d+Bai, c; I/R 7 d+Bai, f), the number of apoptotic cells (arrows; green) was, respectively, obviously increased relative to the sham operation control or decreased in the retinal ganglion cell layer relative to the ischemia and pretreatment with vehicle control. The merged images for TUNEL and DAPI (blue) labeling are shown (a–f). Each bar represents the mean±SEM (n=8) at 1 (g) and 7 days after the operation (h). */†, respectively, represents significant differences (P<0.05) between the vehicle-pretreated ischemic group and the sham operation group and between the vehicle-pretreated ischemic group and the baicalein-pretreated ischemic group. Scale bars=25 μm.