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. 2013 Jul;29(6):539–549. doi: 10.1089/jop.2012.0179

FIG. 7.

FIG. 7.

Real-time polymerase chain reaction analysis of the expression of Thy-1 (a), HIF-1α (b), MMP-9 (c), VEGF (d), HO-1 (e), and β-actin. Twenty four hours after ischemia plus reperfusion (I/R), whole retinal extracts were isolated from the sham operation eyes (control), the ischemic eyes with preadministration of vehicle (1% ethanol), or the ischemic eyes with preadministration of baicalein (0.5 nmol). The effect of the treatments on the ratio of Thy-1/HIF-1α/MMP-9/VEGF/HO-1: β-actin mRNA was determined compared to a control, namely, β-actin, a housekeeping gene. */†, respectively, represent significant differences (P<0.05) between the vehicle-pretreated ischemic group and the sham operation group and significant differences (P<0.05) between the vehicle-pretreated ischemic group and the baicalein-pretreated ischemic group, respectively. The abbreviations are HIF-1α, hypoxia-inducible factor-1α; MMP-9, matrix metalloproteinase-9; VEGF, vascular endothelium growth factor; HO-1, heme oxygenase-1. The results are the mean±SEM (n=6).