Fig. 4.

TCA cycle intermediates. Five of the TCA cycle intermediates were detected with this methodology and were quantified by PARAFAC analysis. The signal value for each metabolite was averaged for each strain, incorporating injection variation and biological variation, and then normalized to wild type. Stearic acid is also provided as a reference as it has been observed to be relatively constant in different growth conditions and strains in a previous study [8]. By ANOVA, α-ketoglutarate, succinate, fumarate, and malate all have statistically significant differences