Table 4.
Statistical comparison by Student’s t test. Each strain pair was compared to identify statistically significant differences. A t-value is provided for each metabolite that differed at the 99.0% confidence level (>2.58) for that pair combination. When the t-value is greater 3.29, this indicates a significant difference at the 99.9% confidence level. For metabolites where the single standard deviation error bars did not overlap, a < or > sign indicates which strain showed larger levels, relative to their order in the column header. For example, in the top left cell, glucose is significantly higher in wt than adrl Δ with a t-value of 4.07
| Metabolite | wt: adrl Δ |
wt: cat8Δ |
wt: adrlΔcat8Δ |
wt: snflΔ |
adrlΔ: cat8Δ |
adrlΔ: adrlΔcat8Δ |
adrlΔ: snflA |
cat8Δ: adrlΔcat8Δ |
cat8Δ: snflΔ |
adrlΔcat8Δ: snflΔ |
|---|---|---|---|---|---|---|---|---|---|---|
| Glucose | 4.07 > | 3.88 > | 2.99 | 7.19 < | 3.26 < | 7.46 < | 3.24 < | 7.44 < | 3.22 | |
| Alanine | 14.05 < | 27.34 < | 3.37 < | 7.00 < | ||||||
| Mannitol | 3.10 | 5.67 < | 4.05 < | 3.75 | ||||||
| Glycine | 2.61 | |||||||||
| 2-Monostearin | 3.11 | 4.03 > | 2.75 | 2.64 | 4.39 < | |||||
| Stearic acid | 2.71 | |||||||||
| Valine | 4.49 < | 4.19 < | 3.13 | |||||||
| Benzoic acid | ||||||||||
| Lysine | ||||||||||
| 3-OH butyric | 6.66 < | 4.55 < | 2.84 | 3.91 | 3.06 | 8.26 > | 6.16 > | |||
| Glycerol | 3.31 | 3.14 | 2.67 | 3.63 < | 3.59 < | 2.62 | ||||
| Glucosamine | 2.67 | 4.36 < | 3.46 | 4.79 < | 5.61 < | |||||
| Glycerate 3 phosphate | 5.62 > | 5.90 > | 3.21 | 8.45 > | 8.86 > | 3.61 | 6.84 > | 7.00 < | ||
| CoA fragment | ||||||||||
| Pyruvate | 3.41 | |||||||||
| CMP | 2.69 | 3.00 | ||||||||
| NADP | 4.31 > | 5.44 > | 4.31 < | 5.03 < | ||||||
| FAD | ||||||||||
| Deoxyadenosine | 5.02 > | 3.84 | 3.32 | 2.97 | ||||||
| Tyrosine | 2.82 | 5.62 > | 2.99 | 4.13 > | 6.06 > | 4.94 > | ||||
| Glycolic | ||||||||||
| Uracil | ||||||||||
| TPP | 5.99 < | 4.87 < | ||||||||
| 3-OH propionic | ||||||||||
| Threonine | 2.83 | 4.02 > | 2.79 | 3.18 | 3.98 > | |||||
| FMN | ||||||||||
| SAM | 3.13 | |||||||||
| Ornithine | 3.37 | |||||||||
| Isoleucine | 4.51 > | 2.53 | 3.97 | 4.22 > | 5.38 > | |||||
| Glycerol 3 phosphate | 4.36 < | 6.09 > | 4.05 < | 3.12 | 3.45 | 6.80 > | 11.91 > | |||
| Orotic | 3.04 | 2.58 | ||||||||
| Cystathionine | 4.36 > | 3.06 | 4.27 > | 4.56 > | ||||||
| Adenine | 3.02 | 3.23 | ||||||||
| SAH | 3.92 > | 6.39 > | 3.26 | |||||||
| Leucine | 5.19 < | 8.28 > | 3.18 | 4.20 > | 4.41 < | 5.58 > | 9.97 > | |||
| Arabinose | 2.60 | 2.96 | ||||||||
| ADP | 16.03 > | 4.40 > | 5.93 > | 7.14 > | 3.63 | 5.40 > | 2.63 | |||
| Threonine | 7.57 > | 4.08 | 2.92 | 2.61 | ||||||
| Phenylalanine | 4.38 > | 4.24 < | 11.65 > | 3.65 | 6.18 > | 6.36 < | 6.30 > | 12.90 > | ||
| Adenosine, 5’S-methyl 5’thio-N- | ||||||||||
| Lactic acid | 2.77 | 2.65 | 3.01 | 3.11 | 4.18 > | |||||
| G1P | 5.31 > | 6.89 > | 5.10 > | 6.59 > | 9.08 > | 5.50 > | 3.58 | |||
| Methionine sulfoxide | 5.67 > | 15.89 > | 3.67 | 3.97 | 4.21 > | 8.91 > | ||||
| methionine | 8.76 > | 2.58 | 7.25 > | 3.69 | 3.70 | 5.63 < | 4.93 > | |||
| Tryptophan | 2.92 | 4.74 > | 2.66 | 5.08 < | 5.65 > | 11.35 > | ||||
| Citrate | 4.14 | 3.48 | 7.20 > | 3.80 | 2.89 | 9.15 > | ||||
| Serine | 6.90 > | 2.85 | 30.65 > | 2.72 | 6.58 > | 3.83 | 5.08 > | 12.22 > | ||
| UDP | 7.38 > | 7.21 > | 5.65 > | 8.60 > | 8.29 > | 6.36 > | 4.07 | 3.54 | ||
| Arginine | 3.74 | 4.59 > | 2.60 | |||||||
| UDP-N-acetylglucosamine | 11.27 > | 13.76 > | 11.19 > | 7.35 > | 8.63 > | 6.87 > | 5.33 < | |||
| Glutamate, 5-oxoproline | 12.80 > | 9.23 > | 18.53 > | 13.78 > | 10.14 > | 19.35 > | 5.54 > | 5.24 > | ||
| Asparagine | 7.77 > | 7.92 > | 8.72 > | 6.49 > | 5.91 > | 6.96 > | 2.93 | 3.49 | ||
| Glutamic acid | 12.04 > | 7.09 > | 16.44 > | 13.90 > | 7.91 > | 18.82 > | 5.66 > | 5.88 > | ||
| UDP/G1P | 9.97 > | 9.53 > | 10.18 > | 10.81 > | 10.28 > | 11.01 > | ||||
| Proline | 6.41 > | 5.26 > | 8.75 > | 5.65 > | 4.75 > | 7.44 > | 5.65 > | 8.16 > | ||
| UDP-glucose | 5.70 > | 6.13 > | 5.57 > | 8.10 > | 8.70 > | 7.82 > | 4.02 > | 3.45 | ||
| Aspartic acid | 19.52 > | 16.89 > | 18.46 > | 3.57 > | 2.58 | 3.78 > | 7.51 < | 6.96 > | ||
| Asparagine | 5.63 > | 3.39 | 6.48 > | 4.00 > | 4.56 > | 4.05 | 5.79 > | |||
| Alpha-ketoglutaric acid | 6.59 > | 6.79 > | 6.23 > | 13.21 > | 13.19 > | 11.78 > | ||||
| G6P | 22.96 > | 21.75 > | 21.72 > | 4.22 > | 4.08 > | 4.10 > | ||||
| Glutamine | 6.11 > | 5.09 > | 6.37 > | 3.17 | 2.74 | 3.30 > | 3.00 | 3.87 | ||
| Succinate | 28.17 > | 21.65 > | 28.87 > | 4.41 > | 4.36 > | 4.59 > | 4.95 > | 5.23 > | ||
| Malate | 5.97 > | 5.94 > | 6.04 > | 4.53 > | 4.50 > | 4.58 > | ||||
| Fumarate | 23.19 > | 23.78 > | 24.42 > | 4.20 > | 4.39 > | 4.66 > | 5.20 > | 2.91 | ||
| Trehalose | 5.60 > | 5.64 > | 6.07 > | 3.61 > | 3.65 > | 4.04 > | 6.36 > | 5.18 > |