Table 1.

Thermodynamic Parameters for Equilibrium Unfolding/Refolding and Kinetic Unfolding of WT HγD-Crys

Equilibrium Unfolding
	t1	t2	m1	m2	ΔG1	ΔG2	ΔΔGt
100 mM NaPia	2.2 ± 0.1	2.8 ± 0.1	3.6 ± 0.1	3.1 ± 0.4	7.7 ± 0.2	8.9 ± 1.3	N/A
250 mM NaCit	2.6 ± 0.1	3.4 ± 0.1	4.1 ± 1.2	3.5 ± 0.7	10.4 ± 3.2	11.8 ± 2.6	5.6

Kinetic Unfolding
	k1	k2	t1	t2
100 mM NaPi	0.0011 ± 7×10−5	0.00017 ± 9×10−6	900 ± 60	6000 ± 300
250 mM NaCit	0.0011 ± 1×10−4	0.00016 ± 2×10−5	910 ± 90	6500 ± 900

^aFrom Flaugh et al., 2005b.