Table 5.
WT HγD-Crys | ||||||
---|---|---|---|---|---|---|
k1 | k2 | k3 | t1 | t2 | t3 | |
|
||||||
100mM NaPi | 0.092 ± 0.003 | 0.017 ± 0.001 | 10.9 ± 0.2 | 60 ± 4 | ||
250mM NaCit | 0.0077 ± 2×10−4 | 0.0016 ± 3×10−4 | 0.00013 ± 2×10−5 | 130 ± 3 | 630 ± 100 | 8200 ± 1400 |
L5S HγD-Crys | ||||
---|---|---|---|---|
k1 | k2 | t1 | t2 | |
|
||||
100 mM NaPi | 0.070 ± 0.003 | 0.00051 ± 7×10−5 | 14.2 ± 0.7 | 1970 ± 280 |
250 mM NaCit | 0.026 ± 0.001 | 0.00061 ± 2×10−5 | 39 ± 2 | 1630 ± 60 |
I90F HγD-Crys | ||||
---|---|---|---|---|
k1 | k2 | t1 | t2 | |
|
||||
100 mM NaPi | 0.0181 ± 2×10−4 | 0.00047 ± 5×10−5 | 55.2 ± 0.6 | 2270 ± 230 |
250 mM NaCit | 0.00184 ± 5×10−5 | 0.00018 ± 2×10−5 | 540 ± 10 | 5730 ± 590 |