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. Author manuscript; available in PMC: 2013 Jul 11.
Published in final edited form as: Exp Eye Res. 2011 May 12;93(4):371–381. doi: 10.1016/j.exer.2011.04.011

Table 5.

Rate of Kinetic Refolding of HγD-Crys

WT HγD-Crys
k1 k2 k3 t1 t2 t3

100mM NaPi 0.092 ± 0.003 0.017 ± 0.001 10.9 ± 0.2 60 ± 4
250mM NaCit 0.0077 ± 2×10−4 0.0016 ± 3×10−4 0.00013 ± 2×10−5 130 ± 3 630 ± 100 8200 ± 1400
L5S HγD-Crys
k1 k2 t1 t2

100 mM NaPi 0.070 ± 0.003 0.00051 ± 7×10−5 14.2 ± 0.7 1970 ± 280
250 mM NaCit 0.026 ± 0.001 0.00061 ± 2×10−5 39 ± 2 1630 ± 60
I90F HγD-Crys
k1 k2 t1 t2

100 mM NaPi 0.0181 ± 2×10−4 0.00047 ± 5×10−5 55.2 ± 0.6 2270 ± 230
250 mM NaCit 0.00184 ± 5×10−5 0.00018 ± 2×10−5 540 ± 10 5730 ± 590