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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Ann Allergy Asthma Immunol. 2013 Jul;111(1):25–31.e1. doi: 10.1016/j.anai.2013.05.010

Figure 3.

Figure 3

Effect of eosinophil co-culture on HRV1A. A, BEAS-2B cells were infected with HRV1A at 20 PFU/cell for 24 hours with or without eosinophils (EOS) as indicated. RNA was extracted from the BEAS-2B cells and RT-PCR performed for viral RNA levels with a standard curve to determine HRV1A RNA levels (PFU equivalents) per μg of cellular RNA. Median HRV1A RNA levels are shown as horizontal bars. P value for statistical comparison is shown. Supernatants were collected for analyses of B, plaque forming units (PFU), and C, copies of HRV RNA from cultures of HRV1A (MOI 1), eosinophils (EOS, 1×106 cells/mL), and fMLP (0.1 mM) as indicated incubated for 3 hours.