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. Author manuscript; available in PMC: 2013 Jul 11.

Published in final edited form as: Doc Ophthalmol. 2009 Jul 29;120(1):25–39. doi: 10.1007/s10633-009-9181-x

a. Lectin stained retinal flat mount of room air raised postnatal day (p)4 rat pup injected with pimonidazole 90 minutes before sacrifice. Lectin stains retina vessels (red) and insoluble pimonidazole conjugates (green) are present in tissue where oxygen concentration is less than 10 mm Hg. Despite nearly 50% of the retina as avascular, little insoluble pimonidazole is present.

b. Lectin stained retinal flat mount of postnatal day (p)18 rat pup from the 50/10 OIR model injected with pimonidazole 90 minutes before sacrifice. Lectin stains retina vessels (red) and insoluble pimonidazole conjugates (green) are present in tissue where oxygen concentration is less than 10 mm Hg. Hypoxic retina (green labeling conjugated pimonidazole) is present in avascular retina and in areas in between vessels within vascularized retina.

a. Lectin stained retinal flat mount of room air raised postnatal day (p)4 rat pup injected with pimonidazole 90 minutes before sacrifice. Lectin stains retina vessels (red) and insoluble pimonidazole conjugates (green) are present in tissue where oxygen concentration is less than 10 mm Hg. Despite nearly 50% of the retina as avascular, little insoluble pimonidazole is present.

b. Lectin stained retinal flat mount of postnatal day (p)18 rat pup from the 50/10 OIR model injected with pimonidazole 90 minutes before sacrifice. Lectin stains retina vessels (red) and insoluble pimonidazole conjugates (green) are present in tissue where oxygen concentration is less than 10 mm Hg. Hypoxic retina (green labeling conjugated pimonidazole) is present in avascular retina and in areas in between vessels within vascularized retina.