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. Author manuscript; available in PMC: 2013 Jul 11.

Published in final edited form as: Doc Ophthalmol. 2009 Jul 29;120(1):25–39. doi: 10.1007/s10633-009-9181-x

a. Lipid hydroperoxide in 50/10 oxygen-induced retinopathy model. Lipid hydroperoxide (LHP) levels in retinas assayed at postnatal days 7, 14, or 18 (p7, p14, p18) from pups injected intraperitoneally (IP) with n-acetylcysteine (NAC) or PBS at postnatal days p2, p6, and p10 in the 50/10 oxygen-induced retinopathy (OIR) model. Overall ANOVA, p<0.001, * p=0.012, Bonferroni post-hoc analysis comparing p18 NAC to p18 PBS injected. (n=8 animals at p7 and 4 animals at p14 and p18).

b. Apocynin treatment significantly reduced percent avascular retina at p18 compared to PBS control. Avascular retinal area and clock hours of intravitreous neovascularization and capillary densities of apocynin treated pups. A: Percent avascular retinal area in 50/10 OIR model after intraperitoneal (IP) injections with apocynin once every 24 h from p12 to p17 and assayed at p18 was significantly reduced compared to phosphate buffered saline (PBS) injected pups (* p=0.017, Student’s t-test; n=11 retinas each). B: Clock hours of intravitreous neovascularization (IVNV) in retinas from 50/10 OIR model after IP injections with apocynin once every 24 h from p12 to p17 and assayed at p18 were not significantly different to PBS injected pups (p=0.26; Mann- Whitney test, n=11).

c. Cleaved caspase-3 expression in the retinas of 50/10 OIR after IP injection with apocynin on p12 and p13 and assayed on p14 was significantly decreased compared to retinas from PBS injected pups (p=0.021, Student’s t-test, n=4). However, no significant difference in cleaved caspase-3 expression was measured in retinas of pups injected with apocynin every 24 h from p12 to 17 and assayed on p18 compared to PBS injected pups (p=0.78, Student t-test, data not shown). (permission from Molecular Vision2007; 13:840–53 http://www.molvis.org/molvis/v13/a92/)

a. Lipid hydroperoxide in 50/10 oxygen-induced retinopathy model. Lipid hydroperoxide (LHP) levels in retinas assayed at postnatal days 7, 14, or 18 (p7, p14, p18) from pups injected intraperitoneally (IP) with n-acetylcysteine (NAC) or PBS at postnatal days p2, p6, and p10 in the 50/10 oxygen-induced retinopathy (OIR) model. Overall ANOVA, p<0.001, * p=0.012, Bonferroni post-hoc analysis comparing p18 NAC to p18 PBS injected. (n=8 animals at p7 and 4 animals at p14 and p18).

b. Apocynin treatment significantly reduced percent avascular retina at p18 compared to PBS control. Avascular retinal area and clock hours of intravitreous neovascularization and capillary densities of apocynin treated pups. A: Percent avascular retinal area in 50/10 OIR model after intraperitoneal (IP) injections with apocynin once every 24 h from p12 to p17 and assayed at p18 was significantly reduced compared to phosphate buffered saline (PBS) injected pups (* p=0.017, Student’s t-test; n=11 retinas each). B: Clock hours of intravitreous neovascularization (IVNV) in retinas from 50/10 OIR model after IP injections with apocynin once every 24 h from p12 to p17 and assayed at p18 were not significantly different to PBS injected pups (p=0.26; Mann- Whitney test, n=11).

c. Cleaved caspase-3 expression in the retinas of 50/10 OIR after IP injection with apocynin on p12 and p13 and assayed on p14 was significantly decreased compared to retinas from PBS injected pups (p=0.021, Student’s t-test, n=4). However, no significant difference in cleaved caspase-3 expression was measured in retinas of pups injected with apocynin every 24 h from p12 to 17 and assayed on p18 compared to PBS injected pups (p=0.78, Student t-test, data not shown). (permission from Molecular Vision2007; 13:840–53 http://www.molvis.org/molvis/v13/a92/)