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. 2013 Jul 15;24(14):2201–2215. doi: 10.1091/mbc.E13-02-0076

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© 2013 Berends et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Localization of the LIN-5 complex at the cell periphery does not require an intact actin or microtubule cytoskeleton. (A) perm-1(RNAi) embryos treated with the microtubule-depolymerizing drug nocodazole or with solvent only (1% DMSO, Control). Embryos were stained for LIN-5, tubulin, and DNA (DAPI). Note that microtubules are severely depolymerized in nocodazole-treated embryos, but that LIN-5 still localizes to the cell periphery (arrows). (B) perm-1(RNAi) embryos treated with the actin-depolymerizing drug latrunculin A, cytochalasin D, or with 1% ethanol (control for cytochalasin D). Embryos were probed with antibodies for LIN-5 and actin. DAPI was used to visualize DNA. Note that LIN-5 is present at the cell periphery of both control and latrunculin A–treated embryos (arrows). (C) Time-lapse images of YFP::GPR-1 embryos permeabilized with perm-1 RNAi and treated with solvent only (Control), latrunculin A, or a combination of latrunculin A and nocodazole. Note that even simultaneous disruption of microtubules and actin does not prevent GPR-1 membrane localization (bottom two sets of panels).