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. 2013 Jul 15;24(14):2216–2227. doi: 10.1091/mbc.E12-12-0883

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© 2013 Yang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — PakB-1-180 mimics the subcellular localization of PakB. (A) Dictyostelium PakB consists of a proline-rich, N-terminal domain, a p21-binding domain (PBD) that recognizes Rac GTPases, an unstructured linker segment, and a C-terminal Ser/Thr protein kinase domain. The positions of PxxP motifs are indicated by black rings. (B) Time-course images of GFP-PakB-1-276 expressed in an aggregation-competent AX3 cell migrating in a cAMP gradient. (C, D) Time-course images of GFP-PakB-1-180 expressed in (C) an aggregation-competent AX3 cell migrating in a cAMP gradient and (D) a growth-phase AX3 cell extending pseudopods and macropinocytic cups. (E) Time-course images of GFP-PakB lacking residues 1–180 (GFP-PakB-Δ1-180) expressed in an AX3 cell. Arrows indicate the direction of migration. Bars, 10 μm.