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. 2013 Jul 15;24(14):2216–2227. doi: 10.1091/mbc.E12-12-0883

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© 2013 Yang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Colocalization of PakB and dAbp1. (A) AX3 cells expressing GFP-PakB-1-180 were fixed and stained using antibodies against dAbp1 and GFP. The overlay shows staining for GFP in green and dAbp1 in red. (B) Images of GFP-PakB-1-180ΔP expressed in PakBˉ cells (top) and GFP-PakB-1-180 expressed in dAbp1-null (dAbp1^–) cells (bottom). Migrating developed cells are shown in the left and middle, and growth-phase cells with macropinocytic cups are shown in the right. (C) RFP-dAbp1 was imaged in a developed migrating AX3 cell (top) and PakBˉ cell (bottom). (D) Expression of GFP-PakB-1-180 in a PakBˉ cell restores the cortical localization of RFP-dAbp1. (E) Expression of constitutively active GFP-PakB-ΔPL, which mislocalizes to the rear of migrating cells, results in recruitment of RFP-dAbp1 to the cell posterior. Arrows indicate the direction of migration. Bars, 10 μm.