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. 2013 Jul 15;24(14):2238–2247. doi: 10.1091/mbc.E13-03-0156

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© 2013 Vitriol et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Cofilin_S3A outcompetes wild-type cofilin for lamellipodia localization. (A) Left, coexpression of cofilin-EGFP and cofilin_S3A-KR. Scale bars, 10 μm. Right, graph depicting the average leading edge profile of cofilin-EGFP before and after CALI of cofilin_S3A-KR (n = 7 cells). After CALI, there is a significant increase in cofilin-EGFP fluorescence. Error bars represent 95% confidence intervals. The graph inset shows a representative kymograph of cofilin-EGFP from a cofilin_S3A-KR CALI experiment. (B) Cells were cotransfected with coronin1B-EGFP and cofilin_S3A-KR. The graph depicts average leading-edge profile of coronin1B-EGFP before and after CALI of cofilin_S3A-KR (n = 6 cells). After CALI, there is a significant loss of coronin1B-EGFP fluorescence from the leading edge. Error bars represent 95% confidence intervals. The inset shows a representative kymograph of coronin1B-EGFP from a cofilin_S3A-KR CALI experiment. Kymographs are pseudocolored to emphasize changes in fluorescence intensity. Time of CALI is indicated with a dotted red line. Kymograph scale bars, 3 μm (vertical) and 3 min (horizontal).