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. 2013 Jul 15;24(14):2269–2284. doi: 10.1091/mbc.E13-02-0088

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© 2013 Ryder et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — PI4KIIα colocalizes with the WASH complex. (A, C) HEK293T cells were either transiently transfected with EGFP-tagged subunits of the WASH complex or stained with MitoTracker green, fixed, and processed for indirect immunofluorescence microscopy. Images were acquired by high-resolution deconvolution microscopy. The z-stack projections are shown. (B, D) Isosurface renderings of the z-stack data created using Imaris software. (E) Calculation of Pearson's correlation coefficient between signal intensities of strumpellin-EGFP, WASH-EGFP, or MitoTracker and endogenous PI4KIIα. Correlation was lost when one channel was rotated by 15°. Here n = 6 for WASH-EGFP, n = 7 for strumpellin-EGFP, and n = 9 for MitoTracker-A488. Statistical comparisons were performed by nonparametric analysis using a group Kruskal–Wallis rank sum test followed by individual Wilcoxon–Mann–Whitney rank sum tests.