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. 2013 Jul 9;10:81. doi: 10.1186/1742-2094-10-81

Figure 1.

Figure 1

NA induces CX3CL1 production by astrocytes. (A) Astrocytes were incubated with control media or NA (1, 10 or 50 μM) for 6 or 24 hours. CX3CL1 levels in the media were assessed by ELISA. ***P <0.001 versus 6-hour control; ΦP <0.05 versus 24-hour control; ΦΦP <0.01 versus 24-hour control. Data are means ± SE of n = 12 replicates per group. (B) Astrocytes were incubated with control media or NA 10 μM for 1, 2, 6 or 24 hours. RNA was isolated and CX3CL1 mRNA levels determined by RT-PCR. Data are expressed as percentage of control values (set to 100%).***P <0.001 versus control. Data are means ± SE of n = 8 replicates per group. C, control; CX3CL1, chemokine (C-X3-C motif) ligand 1; ELISA, enzyme-linked immunosorbent assay; NA, noradrenaline; RT-PCR, reverse transcription polymerase chain reaction; SE, standard error.