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. 2013 Jul 9;10:81. doi: 10.1186/1742-2094-10-81

Figure 2.

Figure 2

In the presence of LPS NA inhibits CX3CL1 production by astrocytes. (A) Astrocytes were incubated with control media, LPS 0.1 and 1 μg/ml alone or in combination with NA 10 μM for 24 hours. CX3CL1 levels in the media were assessed by ELISA. ***P <0.001 versus control; ΦΦΦP <0.001 versus LPS 0.1 μg/ml; θθθP <0.001 versus LPS 1 μg/ml. Data are means ± SE of n = 12 replicates per group. (B) Astrocytes were incubated with control media (white columns), LPS 0.1 μg/ml (black columns) or LPS and NA 10 μM (gray columns) for 1, 2, 6 or 24 hours. RNA was isolated and CX3CL1 mRNA levels determined by RT-PCR. Data are expressed as percentage of control values (set to 100%). ***P <0.001 versus control; ΦΦΦP <0.001 versus LPS. Data are means ± SE of n = 8 replicates per group. C, control; CX3CL1, chemokine (C-X3-C motif) ligand 1; ELISA, enzyme-linked immunosorbent assay; LPS, lipopolysaccharide; NA, noradrenaline; RT-PCR, reverse transcription polymerase chain reaction; SE, standard error.