Table 1.
Primers used for RT-PCR
| Gene name | Forward primer | Reverse primer |
|---|---|---|
| CX3CL1 |
5′-AATCCCAGTGACCTTGCTCATCCA |
5′-TGGACCCATTTCTCCTTTGGGTCA |
| CCL2 |
5′-TGCTGTCTCAGCCAGATGCAGTTA |
5′-TACAGCTTCTTTGGGACACCTGCT |
| CCL6 |
5′-TGTTCCAGCAGGGCATCTTCTTCT |
5′-GCCTCATTTGCATGGAGAGCCATT |
| CCL7 |
5′-GGACCAATTCATCCACTTGCTGCT |
5′-TCTGATGGGCTTCAGCACAGACTT |
| CCL12 |
5′-TGAGTCCTCCAGCTCTCATTCCAA |
5′-TGAACACTGAATCTGGTCCAGCCA |
| CXCL16 |
5′-TGTCGCTGGAAGTTGCTACTGTGA |
5′-TCTTGGACTGCAACTGGAACCTGA |
| IL-1β |
5′-ACCTGCTAGTGTGTGATGTTCCCA |
5′-AGGTGGAGAGCTTTCAGCTCACAT |
| TNFα |
5′-CTGGCCAATGGCATGGATCTCAAA |
5′-AGCCTTGTCCCTTGAAGAGAACCT |
| IFNγ |
5′-AAAGACAACCAGGCCATCAGCAAC |
5′-TCTGTGGGTTGTTCACCTCGAACT |
| COX-2 |
5′-GCATTCTTTGCCCAGCACTTCACT |
5′-TTTAAGTCCACTCCATGGCCCAGT |
| GAPDH | 5′-TGCACCACCAACTGCTTAGC | 5′-GGCATGGACTGTGGTCATGAG |
CCL12 chemokine (C-C motif) ligand 12, CCL2 chemokine (C-C motif) ligand 2, CCL6 chemokine (C-C motif) ligand 6, CCL7 chemokine (C-C motif) ligand 7, COX-2 cyclooxygenase-2, CX3CL1 chemokine (C-X3-C motif) ligand 1, CXCL16 chemokine (C-X-C motif) ligand 16, GAPDH glyceraldehyde 3-phosphate dehydrogenase, IFNγ interferon gamma, IL-1β interleukin-1 beta, TNFα tumor necrosis factor alpha.