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. 2013 Jul 11;9(7):e1003626. doi: 10.1371/journal.pgen.1003626

Figure 1. miR-133a and miR-128 target the 3′ UTR of Prdm16 in HEK293 cells.

Figure 1

(A–B) qPCR analysis of miR-1, miR-206, miR-133a, miR-128 and Prdm16, for asWAT and ingWAT of wildtype mice. (C) Putative miRNA target sites within the 3 ′UTR of Prdm16. (D–E) Luciferase assay. Plasmids carrying luciferase gene linked to 3′ UTR of Prdm16 were cotransfected to HEK293 cells, along with control miRNA, miR-133a (D) or miR-128 (E) at indicated doses. Luciferase activity was measure at 48h post-transfection and normalized. N = 4, *P<0.05, **P<0.01.