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. 2013 Jul 11;9(7):e1003626. doi: 10.1371/journal.pgen.1003626

Figure 4. Genetic ablation of miR-133a promotes the browning and thermogenic gene program in SAT but not BAT.

Figure 4

miR-133a has two alleles, miR-133a1 and miR-133a2. The miR-133a-dKO mice (miR-133a1−/−; miR-133a2−/−) were obtained by intercrossing mice with the genotype of miR-133a1−/−; miR-133a2+/−. The mice (miR-133a1−/−; miR-133a2−/−) were used as a control for the qPCR analysis of BAT, asWAT and ingWAT. (A) miR-133a levels in BAT, (B) brown marker expression in BAT, (C) thermogenic gene program in BAT. (D) miR-133a levels in asWAT, (E) brown marker expression in asWAT, (F) thermogenic gene program in asWAT. (G) miR-133a levels in ingWAT, (H) brown marker expression in ingWAT, (I) thermogenic gene program in ingWAT. The brown markers include Prdm16, Ucp1 and Cidea; the thermogenic genes include Cox8b, Hsl, Atgl and Cpt2. N = 3, *P<0.05, **P<0.01.