Table 1. Adult cardiac parameters measured by micro-ultrasound echocardiography.
| Klf3 H275R point mutant | XS Klf3 gene trap mutant | CH Klf3 gene trap mutant | |||||||
| WT (10) | Het (10) | WT (9) | Het (5) | Homo (7) | WT (6) | Het (6) | Homo (8) | ||
| BW | g | 30±2 | 26±1** | 35±5 | 35±5 | 26.9±0.9 | 34±4 | 34±5 | 29±2 |
| HR | min−1 | 462±8 | 487±11 | 479±20 | 435±19 | 455±13 | 454±21 | 452±18 | 459±17 |
| PV-Ao | cm s−1 | 101±3(5) | 262±24*** (5) | 116±5 | 131±16 | 179±33 | 98±6 | 110±2 | 206±17* |
| CO | ml min−1 | 12.6±0.9 | 22±1*** | 11.8±0.5 | 12±2 | 17±3* | 11.0±0.9 | 10.4±0.9 | 22±1*** |
| LV-ESV | µl | 33±3 | 38±4** | 24±4 | 28±4 | 27±6 | 22±3 | 25±8 | 28±3 |
| LV-EDV | µl | 60±4 | 83±6*** | 50±5 | 54±6 | 65±12 | 46±4 | 48±7 | 76±5* |
| EF | % | 45±3 | 55±2** | 53±4 | 49±6 | 60±3 | 54±4 | 52±7 | 64±3 |
| LVWAd | mm | 0.90±0.04 | 0.93±0.04 | 0.98±0.07 | 1.0±0.1 | 0.88±0.06 | 1.04±0.07 | 1.00±0.07 | 1.01±0.06 |
| LVWPd | mm | 0.75±0.03 | 0.83±0.04 | 0.88±0.05 | 0.91±0.07 | 0.91±0.07 | 0.79±0.02 | 0.78±0.05 | 0.95±0.03 |
| LV E-wave | cm s−1 | 75±1 | 95±1*** (5#) | 69±3 | 69±3 | 68±6(6) | 67±2(5) | 58±5(4) | 78±6(7) |
| LV A-wave | cm s−1 | 54±2 | 53±9 (5#) | 51±3 | 46±3 | 51±4(6) | 43±3(5) | 44±5(4) | 60±6(7) |
| LV E/A | 1.41±0.07 | 2.0±0.4 (5#) | 1.39±0.05 | 1.53±0.09 | 1.33±0.02 (6) | 1.6±0.1 (5) | 1.4±0.1 (4) | 1.3±0.1 (7) | |
| ICT | ms | 9.2±0.6 | 7.1±0.3*(9) | 8.8±0.6 | 10±1 | 9±1(6) | 10±2 | 10±1 | 10±1 |
| IRT | ms | 13.3±0.6 | 10.0±0.4***(9) | 11.1±0.4 | 13±1 | 12.6±0.9 (6) | 13.3±0.7 | 15±4 | 13±1 |
| ET | ms | 48±2 | 53±1* (9) | 50±2 | 51±1 | 51±2(6) | 55±3 | 53.7±0.9 | 51±2 |
| LV Tei Index | 0.48±0.03 | 0.32±0.01*** | 0.40±0.01 | 0.45±0.04 | 0.44±0.04 | 0.43±0.03 | 0.46±0.06 | 0.47±0.03 | |
| LA Area | mm2 | 5.4±0.4 (5) | 8.0±0.4**(5) | 4.3±0.4 | 4.4±0.4 | 5.4±0.8 | 4.4±0.2 | 4.3±0.6 | 7.6±0.8*** (7) |
| PV-PA | cm s−1 | 70±2(5) | 91±3*** (5) | 76±4 | 82±9 | 95±7 | 74±6 | 79±3 | 110±14* |
| RV E-wave | cm s−1 | 27±2 | 36±2*** (6#) | 26±1 | 29±1 (4) | 31±2(6) | 27±3(5) | 26±2 (5) | 40±5**(6) |
| RV A-wave | cm s−1 | 44±1 | 50±4 (6#) | 47±2 | 52±2 (4) | 51±3(6) | 44±4(5) | 44±2 (5) | 54±3(6) |
| RV E/A | 0.61±0.03 | 0.72±0.03* (6#) | 0.55±0.01 | 0.57±0.02(4) | 0.61±0.02*(6) | 0.62±0.04 (5) | 0.59±0.08(5) | 0.74±0.08 (6) | |
| RA Area | mm2 | 7.9±0.4 (5) | 11.1±0.6**(5) | 8.0±0.5 | 9.1±0.8 | 13±2* | 7.7±0.5 | 9±1 | 18±1*** |
| Age | wk | 19±1 | 19±1 | 21±4 | 16±3 | 22±6 | 33±9 | 33±10 | 34±2 |
Mean ± SE, N is shown in parentheses in column heading or in cell if different, missing N was due to fusion of E and A waves (indicated by #) or for other technical reasons.
P<0.001,
P<0.01,
P<0.05 vs. WT; results were compared with age-matched WT mice with same background (usually littermates).
WT, wild type; Het, heterozygous; Homo, homozygous.
BW, body weight; HR, heart rate; PV-Ao, peak velocity in ascending aorta; CO, cardiac output; LV, left ventricle; ESV, end-systolic volume; EDV, end-diastolic volume; EF, ejection fraction; LVWAd, left ventricular wall anterior dimension; LVWPd, left ventricular wall posterior dimension; E-wave, early ventricular filling velocity; A-wave, ventricular filling velocity during atrial contraction; E/A, E-wave to A-wave ratio; ICT, isovolumetric contraction time; IRT, isovolumetric relaxation time; ET, ejection time; Tei Index, myocardial performance index = (ICT + IRT)/ET; LA area, left atrial area; PV-PA, peak velocity pulmonary artery; RV, right ventricle; RA area, right atrial area.