Skip to main content
. 2013 Jul 11;9(7):e1003629. doi: 10.1371/journal.pgen.1003629

Figure 4. Gata4 is required for expression of early gonadal differentiation regulators LHX9 and SF1.

Figure 4

(A–C) Whole-mount immunofluorescent staining for LHX9, SF1, and GATA4 protein in control and Gata4 cKO (Wt1CreER) embryos. Confocal images were taken either sagittally (A) or transversely (B and C). Yellow dashed lines outline coelomic epithelial surface. Yellow brackets indicate thickness of epithelial layer. Scale bars: 50 µm. (D) Quantitative analysis of Gata4, Lhx9, and Sf1 mRNA levels in control and Gata4 cKO (CAG-CreER) urogenital ridges. Plotted here are means ± standard deviation from biological replicates (all values normalized to beta-actin). *, P<0.001 (two-tailed Student's t-test).