Figure 5. Gata4 is not required for expression of WT1 and EMX2 in genital ridge epithelium.

(A and B) Immunofluorescent staining for WT1, EMX2, and GATA4 protein in transverse sections of control and Gata4 cKO (Wt1^CreER;Osr1^CreER) embryos. a, dorsal aorta; ge, genital ridge epithelium; mt, mesonephric tubule; w, Wolffian duct. Scale bars: 50 µm. (C) Comparison of numbers of EMX2-positive cells per 100 µm (along length of epithelial surface) in control and Gata4 cKO (Wt1^CreER;Osr1^CreER) embryos. Cells were counted on transverse sections of embryos taken at similar A-P positions. Plotted here are means ± standard deviation from biological replicates (n = 3 for each genotype). *, P = 0.003 (two-tailed Student's t-test).