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. 2013 Jul 11;9(7):e1003598. doi: 10.1371/journal.pgen.1003598

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© 2013 Baumgartner et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Y2H interactions between Lig, Lig^FG-LA, Rin, Rin^1–175 and Rin^129–492. Lig fused to the DBD interacts with Rin fused to the AD. Lig fused to the AD does not interact with Rin fused to the DBD. Lig binds to the fragment Rin^1–175 containing the NTF2-like domain but not to the fragment Rin^129–492 containing the PxxP motifs. Lig with mutated putative binding sites for Rin (FG repeat mutated to LA repeat; Lig^FG-LA) does not interact with Rin^1–175. (B) A linear protein model of Lig with the predicted UBA domain, the conserved regions (CR2-4) and the FG repeat is presented at the top. Linear protein models of Rin with the NTF2-like domain, the acid-rich region, the PxxP motifs and the RNA-binding motifs (RNA recognition motif (RRM) and arginine-glycine rich region (RRG)) as well as the Rin fragments Rin^1–175 and Rin^129–492 are presented in the middle. A linear protein model of Lig with mutated FG (Lig^FG-LA) repeat is presented at the bottom.