Figure 8. JAK/STAT signaling is activated in lig mutant cells.

(A–C) lig¹ mutant clones (induced with the FLP/FRT system, 72 h old, marked by the lack of lacZ staining) in eye (A and A''), antenna (B and B'') and wing (C and C'') imaginal discs of early third instar larvae. The JAK/STAT signaling reporter 10xSTAT92E-GFP is upregulated in lig1 mutant clones in the posterior side of the eye imaginal disc (A' and A''), antenna imaginal disc (B' and B'') and in the hinge region of the wing disc (C' and C''). Note that the reporter signal is autonomously increased in the mutant clones. Scale bars represent 50 µm. (D) Schematic representation of the interactions shown in this study (left) and a working model of a Lig/Rin/FMR1/Caprin complex (right). Genotypes: (A–C) y w hsFLP/y w; FRT42 arm-lacZ/FRT42 lig¹; 10xSTAT92E-GFP/+.