Table 2.
Real-time PCR primers
|
Primer |
Forward |
Reverse |
Amplicon |
Annealing |
|---|---|---|---|---|
| size (kb) | temp (°C) | |||
| ANP |
TTCCGGTACCGAAGATAACAGCCA |
TGACACACCACAAGGGCTTAGGAT |
91 |
60 |
| BNP |
AGACAAGGGAGAACACGGCATCAT |
ACAGAATCATCTGGGACAGCACCT |
85 |
60 |
| HDAC1 |
TTCCTGCGTTCTATTCGCCCAGAT |
AACAAGCCATCAAACACCGGACAG |
98 |
60 |
| HDAC2 |
TACAACAGATCGCGTGATGACCGT |
TCCCTTTCCAGCACCAATATCCCT |
94 |
62 |
| α-skeletal actin |
TTGTGCACCGCAAATGCTTCTAGG |
GCAACCACAGCACGATTGTCGATT |
90 |
60 |
| α-cardiac actin |
TGTAGGTGATGAAGCCCAGAGCAA |
TGGTGCCAGATCTTCTCCATGTCA |
105 |
60 |
| β-myosin heavy chain |
TGGCTGGTGAGGTCATTGACAGAA |
TGGCTGGTGAGGTCATTGACAGAA |
104 |
60 |
| GAPDH | TGTGATGGGTGTGAACCACGAGAA | CATGAGCCCTTCCACAATGCCAAA | 133 | Per plate |