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. 2013 Jul 11;9(7):e1003613. doi: 10.1371/journal.pgen.1003613

Figure 3. The intronic pA of CSTF3 is involved in feedback autoregulation.

Figure 3

(A) Effect of CstF-77 knockdown by siRNA on expression of CstF-77.L and CstF-77.S mRNAs. Top, time course of the protein level of CstF-77 after knockdown measured by Immunoblot (IB). Data were first normalized to GAPDH and then to the time 0 hr, as indicated below the image. Bottom, the effect of CstF-77 knockdown on the expression of CstF-77.L and CstF-77.S mRNAs. The time points are the same as those in (A). (B) Effect of CstF-77.S knockdown on expression of CstF-77.L. Left, knockdown of CstF-77.S as verified by qRT-PCR (both isoforms 2 and 3 are measured); right, effect of CstF-77.S knockdown on the expression of CstF-77.L. (C) Left, effect of CstF-77 overexpression on the expression of endogenous CstF-77.L and CstF-77.S mRNAs. Right, overexpression of exogenous CstF-77 as verified by IB using an antibody against the Omni tag of exogenous CstF-77. (D) Effect of CstF-77.L and CstF-77.S knockdowns on the P/S ratio of transcripts expressed from the reporter plasmid pRinG-77S-831. (E) Effect of CstF-77 overexpression on the P/S ratio of transcripts expressed from the reporter plasmid pRinG-77S-831. The expression levels of exogenous CstF-77 are the same as those in (C). (F) Time course for expression of endogenous CstF-77.L and CstF-77.S mRNAs and the CstF-77.S/CstF-77.L ratio after plating cells. All values were normalized to those at the 0 hr. All data on mRNA expression were based on RT-qPCR.