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. 2013 Jul 11;9(7):e1003613. doi: 10.1371/journal.pgen.1003613

Figure 4. Intronic C/P of human and mouse CstF-77 genes correlates with the global 3′UTR length.

Figure 4

(A) Analysis of the CstF-77.S/CstF-77.L ratio and global 3′UTR length regulation (RUD) using microarray and RNA-seq data. cUTR, constitutive UTR; aUTR, alternative UTR. Correlation of the CstF-77.S/CstF-77.L ratio with global 3′UTR length was found in 17 human tissues and cell lines (B), 11 mouse tissues (C), and proliferating and differentiating C2C12 cells (D) as indicated by the R2 values (linear regression). (E) Validation of CstF-77.S and CstF-77.L expression in proliferating and differentiating C2C12 cells by RT-qPCR (left). The normalized CstF-77.S/CstF-77.L ratio is also shown (right). P, proliferating; D1, differentiation for 1 day; D4, differentiation for 4 days. (F) Expression of CstF-77 protein in proliferating and differentiating C2C12 cells. The relative amount of CstF-77 is indicated. GAPDH was used as internal control.