Figure 5. The intronic pA of CstF-77 gene is regulated by other C/P factors.

(A) Effect of CstF-64 knockdown by siRNA on the CstF-77.S/CstF-77.L ratio. Top, Immunoblot (IB) showing knockdown efficiency; bottom, CstF-77.S/CstF-77.L ratio as measured by RT-qPCR. P-value (T-test) comparing knockdown and control siRNA samples is shown. (B) As in (A), except that knockdowns of CFI-25 (left), CFI-68 (middle), and CFI-59 (right) are shown. (C) As in (A), expect that knockdowns of CPSF-160 (left) and CPSF-73 (right) are shown. (D) Expression of isoforms P and S from various pRinG-77S-1690 reporter constructs in proliferating and differentiating (1 day of differentiation) cells. The PAS type and DSE type of each construct are indicated (see Figure 2C for detail). The ratio of the log₂(P/S) value from proliferating cells to that from differentiating cells is indicated, where P represents the abundance of isoform P and S the abundance of isoform S. The difference in log₂(P/S) value (proliferating cells vs. differentiating cells) is statistically significant (P<0.05, T-test) for all three constructs.