Table 2. Main effect loci: control of parasite load in spleen and in liver, and visceral pathology.
| Phenotype | Locus | Marker | Genotype | P value | corr. P value | % of expl. variance | ||||||||
| CC | CS | SS | ||||||||||||
| Parasites in spleen | ||||||||||||||
| Both crosses | Ltr3 | D3Mit25 | 0.80 | 4.38±0.16 | (n = 61) | 0.63 | 4.15±0.13 | (n = 108) | 0.48 | 3.87±0.17 | (n = 62) | 0.094 | NS | NA |
| (BALB/c×CcS-16)F2 | Ltr3 | D3Mit25 | 1.72 | 5.15±0.22 | (n = 29) | 0.75 | 4.32±0.21 | (n = 43) | 0.43 | 3.76±0.22 | (n = 37) | 0.00014 | 0.0085 | 19.38 |
| (CcS-16×BALB/c)F2 | Ltr3 | D3Mit25 | 0.38 | 3.63±0.21 | (n = 32) | 0.49 | 3.89±0.15 | (n = 65) | 0.61 | 4.11±0.23 | (n = 25) | 0.304 | NS | NA |
| Parasites in spleen | ||||||||||||||
| Both crosses | Ltr6 | D11Mit37 | 0.57 | 4.04±0.16 | (n = 65) | 0.46 | 3.84±0.12 | (n = 105) | 0.96 | 4.56±0.16 | (n = 62) | 0.0028 | 0.113 | NA |
| (BALB/c×CcS-16)F2 | Ltr6 | D11Mit37 | 0.65 | 4.17±0.24 | (n = 31) | 0.45 | 3.81±0.21 | (n = 46) | 1.75 | 5.17±0.23 | (n = 32) | 0.00024 | 0.014 | 29.58 |
| Splenomegaly | Ltr8 | D18Mit49 | 5.28 | 1.70±0.06 | (n = 74) | 4.67 | 1.57±0.05 | (n = 106) | 3.60 | 1.30±0.07 | (n = 53) | 0.00022 | 0.012 | 18.59 |
| Parasites in liver | Ltr2 | D2Nds3 | 0.61 | 4.12±0.11 | (n = 60) | 0.83 | 4.42±0.08 | (n = 123) | 1.25 | 4.83±0.14 | (n = 49) | 0.00056 | 0.028 | 9.50 |
| Hepatomegaly | Ltr2 | D2Mit389 | 45.76 | 37.41±0.81 | (n = 55) | 42.28 | 34.66±0.52 | (n = 131) | 48.31 | 39.42±0.86 | (n = 46) | 4.3×10−6 | 0.00033 | 13.83 |
Parasite numbers (week 43) were estimated by PCR–ELISA. Means, SE and P values for splenomegaly (week 43), hepatomegaly (week 43) and concentration of parasite DNA (ng/µl) in isolates from lymph nodes, spleen and liver were calculated by analysis of variance. Normal distribution was obtained for splenomegaly (spleen-to-body weight ratio×1000) by raising values to the power of 0.00002. Hepatomegaly (liver-to-body weight ratio×1000) was normalized by raising values to the power of 0.0125. To obtain normal distribution for parasite load in organs, the following transformations were used: natural logarithm of (value×100). The numbers in bold give the average non-transformed values. Only P values significant after correction for genome-wide testing are given. Number of tested mice is shown in brackets. C and S indicate the presence of BALB/c and STS allele, respectively. NS – not significant, NA – not applicable.