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The activity against the substrates is represented in the y axis relative to the highest activity of the library, whereas the x axis shows the different amino acids using the one-letter code (n = norleucine). Error bars display the standard deviation from triplicate experiments. (A) FheCL3, (B) FheCL3 H61N (C) FheCL3 W67L (D) FheCL3 H61N W67L.
