Table 2. Comparison of binding to Dyn2 and tetherin for Nef substitutions with impaired infectivity enhancement.

	Infectivity enhancementa	Dyn2 bindingb	BST2 bindingc
Nef	1	1.1	1
G2A	0.81	0.17	0.16
64–65	0.39	0.35	0.54
66–67	0.43	0.46	0.18
68–69	0.27	0.50	0.14
82–83	0.51	0.26	1.38
94–95	0.58	0.17	NT
98–99	0.49	0.50	NT
112–113	0.59	0.18	0.35
146–147	0.50	0.35	1.56
168–169	0.54	0.52	2.1
195–196	0.42	0.22	0.31

^aInfectivity enhancement was calculated as the relative infectivity of Δnef-viruses trans-complemented with these Nef mutants to Δnef-viruses trans-complemented with wild-type Nef.

^bBinding to dynamin 2 was calculated as the relative band intensity of Nef to the band intensity of dynamin 2 in immunoprecipitated samples.

^cBinding to tetherin was calculated as the relative band intensity of Nef to the band intensity of tetherin in immunoprecipitated samples.