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. 2013 Jul 11;9(7):e1003494. doi: 10.1371/journal.ppat.1003494

Figure 2. Effect of ZAP-S on viral promoter activities.

Figure 2

(A) Expression of ZAP-S does not alter HBV core promoter activity in transfected cells. HepG2 cells were seeded in a 96-well-plate and cotransfected with 100 ng of EnII/Cp-Luc and 4 ng of pRL-CMV, plus 100 ng of control vector or plasmid ZAP-S. Three days after transfection, cells were harvested and luciferase activities were measured. The plotted relative luciferase activity (RLA) represents the mean ± standard deviation (SD, n = 4) of the ratios of absorbance obtained from wells expressing ZAP-S over that obtained from wells that were transfected with control vector. (B) ZAP-S overexpression enhances HBV surface promoter activity. HepG2 cells in 96-well-plate were transfected with 100 ng of S1-Luc or S2-Luc, together with 100 ng of control plasmid or ZAP-S expressing vector. 4 ng of pRL-CMV was included in each transfection for the normalization of transfection efficiency. Luciferase assays were performed 3 days post transfection.