Table 1. pH and electrical potential in each compartment of the E. coli reconstruction iAF1260.

Compartment	pH	Electrical potential (mV)
Cytosol	7.70	0
Periplasm	7.70	90
Extracellular fluid	7.70	90

Electrical potential in each compartment is relative to electrical potential in the cytosol. Temperature was set to 310.15 K (37°C), and ionic strength was assumed to be 0.25 M [14] in all compartments. Taken from [8].