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. 2013 Jul 11;9(7):e1003495. doi: 10.1371/journal.ppat.1003495

Figure 4. An oligomerization deficient YscF mutant.

Figure 4

(A) Schematic of native YscF and YscF35/67 mutants. (B) Purification of YscF and YscF35/67 mutant proteins. The gel filtration profiles showed that the native YscF eluted as a broad peak spanning the entire high molecular weight range and the mutated YscF35/67 eluted as two peaks, one as a high molecular weight aggregate near the void volume, and another at 22 kDa corresponding to the size of a dimer. (C) Purity of YscF and YscF35/67 proteins as analyzed by SDS-PAGE and Coomassie blue staining of the peak fractions.