Figure 3. Surface location in E. coli of the autotransporter LasA_I as determined by immuno-dot blot analysis.

Serial dilutions of E. coli cells were deposited onto a nitrocellulose membrane. The presence of LasA_I was detected with anti-LasA_I antibody. A: E. coli containing control plasmid pET102; B: IPTG-induced E. coli containing recombinant plasmid pET102-lasA _I; C: IPTG induced E. coli containing recombinant plasmid pET102-lasA _I treated with Proteinase K.