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Serial dilutions of bacterial cells were deposited onto a nitrocellulose membrane; GFP was detected with anti-GFP antibody. Lane 1, 2, 3: IPTG induced E. coli containing plasmid pET102-gfp; Lane 4, 5, 6: IPTG-induced E. coli containing plasmid pET102-gfp-lasA I-TD; Lane 7, 8, 9: IPTG induced E. coli containing plasmid pET102-gfp-lasA II.
