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. 2013 Jul 11;8(7):e68293. doi: 10.1371/journal.pone.0068293

Figure 1. Side population (SP) cell sorting results and sorting purity in human renal cell cancer cell line 769P.

Figure 1

A, SP sorting using Hoechst 33342. A polygonal live gate was created to exclude debris and dead cells. At least 50,000 cells were acquired to analyze the SP phenotype of every sample. The percentage of SP cells dropped when 769P cells were pre-incubated with verapamil to block the ATP transporter. B, the purity of freshly sorted 769P SP and non-SP (NSP) cells was 96.61% and 99.89% (1–0.11%).