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. 2013 Jul 11;8(7):e68293. doi: 10.1371/journal.pone.0068293

Figure 2. Clone formation and long-term culture differentiation of sorted 769P SP and NSP cells.

Figure 2

A, clone formation assays reveal that the clone formation efficiency of freshly sorted SP cells was higher than that of NSP cells. **, P<0.01. B, at 10 days after sorting, the proportion of SP cells (indicated by the R2 gate) among sorted SP cells and sorted NSP cells were measured. The proportion of SP cells among sorted SP cells is only 19.51%, suggesting that most sorted SP cells are differentiated into NSP cells. The proportion of SP cells among sorted NSP cells is only 2.39%, suggesting that sorted NSP cells cannot differentiate into SP cells.