Figure 3. Expression of ABC family members in sorted 769P SP and NSP cells.

A, reverse transcription-polymerase chain reaction. B, Western blotting. Lane 1, sorted NSP cells; lane 2, sorted SP cells. GAPDH was used as the internal control. Both experiments show that ABCB1 expression is stronger in SP than in NSP cells and that ABCG2 and ABCC1 are not expressed in SP and NSP cells.