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. 2013 Jul 11;8(7):e68384. doi: 10.1371/journal.pone.0068384

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Motility of ΔflhB Salmonella strain transformed with plasmids coding for different variants of FlhB. The asterisk “*” marks the previous emplacement for the wild type strain that was removed because it would have overgrown after 46 hours. (B) Rescue of motility by mutations in the cytoplasmic domain of FlhB. The plates were incubated at 30°C for the indicated time. Indicated plasmids carry genes of the following proteins: wild-type SalFlhB (WT); AquFlhB (pTAB32); Sal/AquFlhB (pTB334); Sal/AquFlhB347 (pTB335); Sal/AquFlhB357 (pTB336); Sal/AquFlhB V341 frame-shift (pTB337); Sal/AquFlhB F343 frame-shift (pTB342); Sal/AquFlhB357 V259E (pTB341); Sal/AquFlhB347 F330L (pTB338); Sal/AquFlhB347 V307G (pTB339); Sal/AquFlhB347 ΔKG(288–289) (pTB340).