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Cells carrying the plasmids encoding different FlhB proteins were grown at 37°C until OD600 = 0.5–0.6. Protein expression was induced by 0.1 mM IPTG. After 2 hours cells were harvested by centrifugation and analyzed by immunoblotting using polyclonal antibodies against full-length Aquifex and Salmonella FlhB.
