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. 2013 Jul 11;8(7):e68384. doi: 10.1371/journal.pone.0068384

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Cells were transformed with pTrc99A-based plasmids encoding various FlhB proteins. Soft agar plates were incubated at 30°C for the indicated time. (A) Motility of ΔflhB Salmonella strains producing Sal/AquFlhB F343 frame shift. One of the strains contains additional mutation in fliS. 1 - empty vector, 2 - wild type Salmonella FlhB, 3 - Sal/AquFlhB F343 frame shift, 4 - Sal/AquFlhB F343 frame shift plus FliS (A22T). The asterisk “*” marks the previous emplacement for the wild type strain that was removed because it would have overgrown after 20 hours. (B) Comparison of the motility of ΔflhB and ΔflhB fliS(A22T) Salmonella strains producing different FlhB variants.