Table 4. Oligonucleotides used in the strain and plasmid constructions.
Primer name | Sequence (5′ to 3′)a |
5′-Sal-flhB | CATATGGCAGAAGAGAGCGACGAC |
3′-Sal-flhB-1-218 | GACATCATTATCTTTTTCAGGTGGCTAAAGATCTGG |
5′-Aqu-flhB-213-350 | CTTTAGCCACCTGAAAAAGATAATGATGTCGAGAAGGGAATTG |
3′-Aqu-flhB | CTAGAGGATCCTATTAGGCGTAAACC |
5′-pMM26-Linear-347 | GTCTGGCAGCTTAAACGCTGG |
3′-pMM26-Linear-218 | TTTCAGGTGGCTAAAGATCTGG |
5′-Aqu-flhB-213 | TTTAGCCACCTGAAAAAGATAATGATGTCGAGAAGGG |
3′-Aqu-flhB-340 | TTTAAGCTGCCAGACGTAGGCTATTATTTCCGCTACGG |
5′-pMM26-Linear-357 | GGCGGGCAACGTCCTCCAC |
3′-Aqu-flhB-350 | AGGACGTTGCCCGCCGGCGTAAACCTTTTTCTTTTTGAAC |
Restriction sites are underlined.