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. 2013 Jul 11;8(7):e68301. doi: 10.1371/journal.pone.0068301

Figure 5. VIM knock-down using siRNA.

Figure 5

A: Western blot analysis of VIM in non-transfected TALH-STD and TALH-STD cells transfected with the VIM siRNAs 1, 2, 3 or all three combined. B: siRNA transfected TALH-STD-cells and TALH-STD were subjected to either to NaCl or glucose stress. Cell viability and proliferation, determined with MTT assay, showed a slight increase in cell proliferation upon siRNA treatment but the transfected cells showed higher sensitivity to osmotic stress compared to control. C: The monitoring of apoptosis in siRNA TALH-STD-cells subjected to osmotic stress was carried out using Western blot for caspase 8 (left panel) and 3 (right panel). Each bar represents means ± S.D. of results from 3 independent experiments. Significant differences: (*) p<0.05, (**) p<0.01, (***) p<0.001.