Figure 2. Down regulation of PbGP43 does not affect viability or vitality of transformed P. brasiliensis yeast cells.

Yeast-phase P. brasiliensis PbWt, PbEV and PbGP43 aRNA1 were evaluated by (A) culture turbidity was determined at Optical Density 600 nm and (B) culture medium pH during in vitro growth in BHI. PbWt yeast cells treated with 16 µg/ml of amphotericin B during 4 hours was used as negative control of the assay. The assays were performed in triplicate.