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. 2013 Jul 11;8(7):e68434. doi: 10.1371/journal.pone.0068434

Figure 3. PbGP43 aRNA mutants showed reduced levels of secreted gp43.

Figure 3

(A) Silver stained poliacrylamide gel of culture supernatant (day 12) from PbWt and mutant PbGP43 aRNA1 at the indicated volumes. P27 low-expression strain (PbP27 aRNA) was used as Pb339 recombinant control. (B and C) Immunoblots of culture supernatants tested with anti-gp43 polyclonal and monoclonal antibodies. Culture supernatant volumes are indicated. Purified native gp43 (50 ng) was used as control. (D) Silver stained SDS-PAGE gel of P. brasiliensis culture supernatants from strains PbWt, PbEV and PbGP43 aRNA1. (E and F) Ligand affinity assays with the samples analysed in (D), (E) laminin, and (F) fibronectin (30 µg/ml). Purified native gp43 (50 ng) was used as control.