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. 2013 Jul 11;8(7):e68434. doi: 10.1371/journal.pone.0068434

Figure 4. Down regulation of PbGP43 expression correlated with decreased recovery of intracellular/adhered yeasts from murine MH-S macrophages.

Figure 4

(A) Recovered CFU from lysed IFN-γ activated macrophages; (B) Recovered CFU from supernatants of P. brasiliensis-infected culture macrophages; (C) gene expression levels of PbGP43 (relative to TUB2) during macrophage interaction assays with PbWt, PbEV, and PbGP43 aRNA1 yeasts. The periods evaluated were 0, 1, 3, 6, 12, 24 and 48 hours after interaction (*, p<0.05 when compared with PbWt); (D) TNF-α gene expression (relative to UBI1). The assays were performed in triplicates. Error bars indicate standard deviation.