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. 2013 Jul 11;8(7):e67296. doi: 10.1371/journal.pone.0067296

Figure 5. Induction of APO expression in HLCs derived from hESCs and hiPSCs.

Figure 5

(A) Analysis of apolipoprotein A1, A2, A4, C3, E and LDLR mRNA expression by qRT-PCR (for cell-type nomenclature see Fig. 3A legend). Note that with the exception of APOB (LDL particles), APOC3 (VLDL particles) and APOE (all particles) all other apolipoproteins are part of HDL particles. Error bars represent the standard error of the mean. (B) Apolipoprotein expression by quantitative immunofluorescence. WK1HLCs were labeled with anti-human ALB and either anti-human APOA1, APOA2, APOC3 or LDLR antibodies as described and analysed (see methods and materials). ALB expression was detected through a mouse anti-goat Alexa 594 conjugated secondary antibody (red) and the apolipoprotein expression was detected through a mouse anti-rabbit Alexa 488 conjugated secondary antibody. Insets depict representative high resolution images showing apolipoprotein (green) and albumin (red) expression in the top panels and DAPI (blue) in the bottom panels. Error bars represent the standard deviation.