Figure 1. The chromatogram of crude protein extracted in the leaf of G. procumbens.

The gel filtration separation was performed using HiPrep 16/60 Sephacryl S-100 HR column. The protein fractions were eluted with 50 mM sodium phosphate, pH 7.2, and flow rate of 0.5 mL/min. The active protein fractions 11 and 12 were eluted out within 50 mL to 60 mL of elution buffer.